Paper Title :Bioremediation by Single Culture of Pseudomonas Putida in Petroleum Contaminated Soil
Author :M. A. Nogo Ole, A. Tuhuloula, S.R. Juliastuti
Article Citation :M. A. Nogo Ole ,A. Tuhuloula ,S.R. Juliastuti ,
(2017 ) " Bioremediation by Single Culture of Pseudomonas Putida in Petroleum Contaminated Soil " ,
International Journal of Advances in Science, Engineering and Technology(IJASEAT) ,
pp. 81-85,
Volume-5,Issue-1, Spl. Iss-2
Abstract : Land contamination by petroleum exploration, production and discharge of waste causing serious damage to the
ecosystem of the environment, human and animal. Biodegradation (bioremediation) processes have difficulty focused due to
the complexity of the hydrocarbons that was adsorbed by the soil. The objective of this research was to determine the
efficiency of bioremediation as a function of bacterial concentration. A method that was developed in bioremediation of
petroleum-contaminated soil in addition to in-situ bioremediation was the ex-situ bioremediation with slurry phase
bioreactor. The mixture of soil and water were fed in the bioreactor. The aerobic bacteria used in biodegradation of benzene,
toluene, and xylene (BTX) process were Pseudomonas putida. The variables that would be measured were BTX
concentration; bacterial population and operating conditions (temperature, pH and dissolved oxygen). Residues of petroleum
hydrocarbon (BTX) were measured by gas chromatography method. Process was identified during 56 days in 3 bioreactors
in parallel. Reactor was added by 12,5% (v/v) (A), 15% (v/v) (B)and 17,5% (v/v) (C) Pseudomonas putida bacteria. The
bioreactors were agitated and aerated during bioremediation process. After 56 days, this research resulted in total
degradation of BTX in reactor A reached 91,0400%; reactor B 91,1489%; and reactor C 97.6726%.
Keywords- Pseudomonas putida; bioremediation; Benzene; Toluene; Xylene; slurry bioreactor
Type : Research paper
Published : Volume-5,Issue-1, Spl. Iss-2
DOIONLINE NO - IJASEAT-IRAJ-DOIONLINE-7208
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Published on 2017-05-09 |
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