Paper Title :A New Pyrylium Compound for Sensitive Protein Assay
Author :Mohamed K. Al-Essa
Article Citation :Mohamed K. Al-Essa ,
(2017 ) " A New Pyrylium Compound for Sensitive Protein Assay " ,
International Journal of Advances in Science, Engineering and Technology(IJASEAT) ,
pp. 13-14,
Volume-5, Issue-3, Spl. Iss-1
Abstract : Promising pyrylium compound for use in assaying protein into a wide range (0.606-200nMolar) with very high
sensitivity is presented here in. The compound is having very low fluorescence intensity when it is unbound. However, when
reacting with an amine, the compound is emitting very high fluorescence. Samples containing amine including proteins can
be assayed with high accuracy by using this pyrylium compound. The compound was checked with different concentrations
of bovine serum albumin (BSA) and proved reproducible for protein estimation. The lowest amount that had been detected
in the corresponding samples was 40ng of BSA/ml. Results are showing concentration dependent increase in fluorescence
intensity and standard concentration curves can be generated for estimating unknown protein concentrations with high
linearity (R2 >0.96 ). The compound would be promising for development as kit for high sensitive estimation of protein
concentration in samples.
Type : Research paper
Published : Volume-5, Issue-3, Spl. Iss-1
DOIONLINE NO - IJASEAT-IRAJ-DOIONLINE-9033
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Copyright: © Institute of Research and Journals
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Published on 2017-10-21 |
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